Serotonin Release Assay *

SIM

LAB3201

CPT

82542

Synonyms

SRA; Functional HIT Assay; Heparin-dependent Platelet Antibody

Specimen Type

Serum, frozen

Container

Red-top or gold-top tube

Collection Instructions

None

Volume

Volume: 1 mL
Minimum volume: 0.5 mL 

Additional Information

Confirmatory assay used in the evaluation of heparin induced thrombocytopenia. 

Patient Preparation

Specimen Processing Instructions

Once in the lab, separate serum from cells, transfer serum to a plastic transfer tube and freeze ASAP

Transport Requirements

Frozen

Specimen Stability

Frozen: 6 months 

Rejection Criteria

Room temperature specimen received; grossly hemolyzed, icteric, and lipemic samples

Reference Range

Reference range accompanies report

Critical Value

Test Includes

Serotonin Release Assay  

Methodology

Detects activation of washed donor platelets by measuring the endogenous serotonin induced by addition of patient serum. The serotonin levels are measured by highly sensitive liquid chromatography/tandem mass spectroscopy method. Therapeutic levels of heparin in the assay mix allow for the formation of PF4/heparin complexes on the donor platelet surface to provide an antigen target for the HIT antibodies. The specificity of the assay is increase by assessing the inhibition of platelet activation by adding heparin in excess, thus providing the heparin dependency of the serotonin release.

Testing Frequency

LCA

150018 

Reviewed By

Rhonda Crowe

Reviewed Date

3/1/23

Supplemental

* Denotes Reference Lab Test