Lab Test Details
Lab Test Details
| SIM | LAB3189 |
| CPT | 86480 |
| Synonyms | TB Gold; Quantiferon® TB Gold; Interferon-gamma Release Assay for Tuberculosis |
| Specimen Type | Whole blood- special tubes from lab. |
| Container | The QuantiFERON®-TB Gold Plus collection kit contains the instructions for the collection and handling of (one each): (1) gray-top (with white ring), uncoated (nil); (2) green cap with white ring, TB1 tube; (3) yellow cap with white ring, TB2 tube; (4) purple top with white ring, mitogen-coated. A high altitude kit is also available for locations between 3350 and 6150 feet; a cap with a yellow ring differentiates the tubes. |
| Collection Instructions | Because of limited vacuum in these tubes, use a needle and holder (not a butterfly). If a butterfly is required, first collect other required tubes or use another Vacutainer™ tube to purge the butterfly line of air and then proceed with drawing. Fill tubes to the black fill line on the tube. Do not overfill or under fill. If this happens, immediately redraw a replacement tube. Following proper fill, shake tubes 10 times firmly enough to ensure the entire surface of the tube is coated with blood cells to solubilize the antigen on the tube walls. Place properly filled and labeled tubes back in box. Do not seal the box containing the tubes before delivering to the lab. Do not centrifuge or refrigerate! Specimen can only be collected Sunday –Thursday. Specimens cannot be collected the day before a holiday. |
| Volume | 1 mL x four tubes (see previous instructions) |
| Additional Information | Used to aid in the diagnosis of both latent and active infection with Mycobacterium tuberculosis. This test has been shown to be accurate in HIV-positive individuals with moderately advanced disease. |
| Patient Preparation | |
| Specimen Processing Instructions | LAB Processing ONLY: DO NOT Centrifuge. Four tubes will come in a box, (QFT™ kit ) check each tube to ensure each is properly filled and labeled correctly. Place tubes back in the box. Shake for a few seconds. Attach a lab computer label on the box and place in the incubator (37▫) standing upright in the Microbiology Department overnight.(16-24 hrs.) Leave information for Lab Corp processor on the log sheet. QuantiFERONs cannot be sent via the pneumatic tube system. |
| Transport Requirements | Before incubation at 37°C, maintain specimen at room temperature. Stability: Kit must be incubated within 16 hours of collection. |
| Specimen Stability | Stability: Kit must be incubated within 16 hours of collection. |
| Rejection Criteria | Specimens not collected in special tubes. Centrifuged, refrigerated or frozen specimens. Tubes not labeled correctly. |
| Reference Range | Negative |
| Critical Value | |
| Test Includes | Quantiferon® Plus TB |
| Methodology | Whole blood interferon-gamma test measuring responses to ESAT-6 and CFP-10 antigens. |
| Testing Frequency | |
| LCA | 182893 |
| Reviewed By | Rhonda Crowe |
| Reviewed Date | 2/27/23 |
| Supplemental | * Denotes Reference Lab Test |