Platelet Function Assay

SIM

20030 

CPT

85576 

Synonyms

PFT – Platelet Function Test;  PFA 

Specimen Type

Sodium Citrate Whole Blood and EDTA Whole Blood 

Container

4.5 ml Sodium Citrate 3.2% Blue stopper tube or if the 1.8 ml size sodium Citrate tube is drawn, 2 tubes are needed and one 3.0 ml EDTA Lavender stopper tube (Whole Blood).

Collection Instructions

DO NOT SEND SAMPLES THRU THE TUBE SYSTEM AFTER COLLECTION

Volume

4 ml Whole Blood 
Min: 2.0 ml 

Additional Information

HCT AND Platelet must be within acceptable testing range to perform assay.

ACCEPTABLE TESTING RANGE: HCT = >35% and Platelet Count = >150,000/ uL

Patient Preparation

none

Specimen Processing Instructions

Store Whole Blood at room temperature.  Do Not Centrifuge.

Transport Requirements

Room Temperature

Specimen Stability

Room Temperature Whole Blood: 4 hours  

Rejection Criteria

• Clotted Sample 
• Insufficient volume 
• Plasma removed 

Reference Range

PFA Collagen / Epinephrine = 75 - 184

PFA Collagen / ADP = 44 - 142

Critical Value

None 

Test Includes

If results of the PFA Coll-Epi are greater than the reference range, we will test the sample using the test cartridge PFA Coll-ADP. 

Methodology

The PFA-100 is an instrument and test cartridge system in which the process of platelet adhesion and aggregation following a vascular injury is simulated in vitro.   

Testing Frequency

 Monday - Sunday 

Reviewed By

Crystal Killian

Reviewed Date

Supplemental