Bilirubin Profile

SIM

10492 

CPT

82247; 82248 

Synonyms

Bilirubin Fractionization   D and T Bili Profile  Indirect Bilirubin 

Specimen Type

Plasma or Serum 

Container

3.5 ml Green - Lithium heparin plasma separator 
4.0 ml Gold-top serum separator

Collection Instructions

None

Volume

2 ml plasma or serum 
Min: 0.5 ml

Additional Information

Indirect Bilirubin = Total Bilirubin minus Direct Bilirubin

Patient Preparation

None

Specimen Processing Instructions

Plasma:  Centrifuge at 3380 rpm’s (±100) for a minimum of 10 minutes 
Serum:  Allow to clot.  Centrifuge at 3380 rpm’s (±100) for a minimum of 10 minutes 
Centrifuge within 2 hours of collection. 

Transport Requirements

Refrigerated

Specimen Stability

Room temperature:  8 hours   Refrigerated: 5 days 

Rejection Criteria

•  If not centrifuged within 2 hours of collection. 
•  Gross Hemolysis

Reference Range

Direct Bili = 0.0 – 0.2 mg/dl 
Indirect Bilirubin = 0.2 - 1.4 mg/dl 
Total Bilirubin = Adult range = 0-1.5 mg/dl 
Neonatal Total Bilirubin Ranges: 
0 min. - 1 day = 0-6.0 mg/dL 
1 day - 2 days = 0-8.0 mg/dL 
3 days - 10 days = 0-12.0 mg/dL 
11 days - Adult = 0-1.5 mg/dL

Critical Value

Neonate Total Bilirubin critical = >12.0 mg/dl 

Test Includes

Direct Bilirubin   Indirect Bilirubin = Total Bilirubin minus Direct Bilirubin  Total Bilirubin 

Methodology

Timed Endpoint Diazo method 

Testing Frequency

Daily, Monday - Sunday 

Reviewed By

Crystal Killian 

Reviewed Date

3/17/2021

Supplemental